Plant growth promoting bacteria are the group of bacteria that colonize the rhizosphere and exert a beneficial impact on plant health and soil fertility. Microbes can directly facilitate the plant growth in several ways such as syntheses of phytohormones, enzymes or indirectly by protecting from pathogen via antagonistic activity and by minimizing effect of abiotic stress. Present study was carried out with the sample isolated from rhizosphere of plant, Chlorophytum borivilianum. Isolates that shows positive activity any of N2 fixation, phosphate solubilisation, IAA production were studied for their antagonistic activity against fungi, pot trials on wheat and RAPD of isolates was done to show relativeness. Four isolates showed antifungal activity toward plant pathogen, Fusarium oxysporum. Out of these four isolates D3 showed highest antagonistic activity against F. oxysporum with 89% in term of percentage inhibition. The combination of isolate A3 & D3 significantly enhance the overall growth of wheat. UPGMA cluster method was used to generate phylogenetic tree. Study can encourage farmers to use PGPR to enhance crop productivity.