Seasonal variation of soil mycoflora isolated from two forest ranges viz. Motichur and Chilla of Rajaji national park, Uttarakhand were studied for a period of twenty four months by using serial dilution agar plate technique. Results revealed that the higher CFU s (colony forming units) of mycoflora were recorded from Motichur forest range during rainy season followed by summer and winter season. Total 20 fungal species were reported and about 19 different species belongs to three groups viz. Ascomycotina, Zygomycotina and Deuteromycotina were identified with the help of relevant literatures and one was unidentified. The fungi isolated include one species of Acomycetes, one species of Zygomycetes and remaining species were Deuteromycetes. A marked seasonal variation in mycoflora has been found. Fungal flora was also correlated with varying ecological factors viz. temperature, moisture content, pH, organic carbon and nitrogen.