In vitro pollen germination test was performed on Mitragyna parvifolia (Roxb.)Korth. belonging to the family Rubiaceae to study the effect of different nutrients like sucrose, boric acid at various concentration separately and in combinations and salts like Calcium nitrate, Magnesium sulphate and Potassium nitrate. The flowers open in the early evening 19.00 to 20.30 hrs. Anther dehiscence takes place before flower opening. Maximum 96% pollen germination along with 1105µm pollen tube development was observed in 10% sucrose solution supplemented with 100ppm boric acid and among the salts maximum 90% pollen germination along with 910µm pollen tube was observed in 50ppm Calcium nitrate solution and in case of Magnesium sulphate 85% pollen germination along with 728µm pollen tube was observed in 500ppm while in case of Potassium nitrate 65% pollen germination along with 897µm pollen tube was observed in 500ppm solution.