The study aimed to see the ability of some actinomycetes in removing of some toxic heavy metal ions (Cd²⁺, Mn²⁺) and some aflatoxins (AFG1, AFG2, AFB1, AFB2) from contaminated water samples. Out of 57 isolates of actinomycetes were isolated from different locations of Egypt. The most five isolates removed studied heavy metals, (Cd²⁺, Mn²⁺) singly above 65% were selected to remove studied metal ions from binary mixtures. The highest removing percentage was recorded by A26 isolate which was removed 71.4 %, and 70.0 % from Cd²⁺, and Mn²⁺ respectively. The 16S rRNA analyses and phylogenetic data of A26 concluded that it was member of Streptomyces genus, A26 was deposited in Gene Bank Data base under accession number MW947066. The results of the dead biomass of Streptomyces albus A26 for Cd²⁺, and Mn²⁺ under the optimized conditions pH-8 at 40°C for 3 hours with 0.3% bio sorbent dosage was found (Cd²⁺79.5 ± 2.37%, Mn^{+ 2,}81.6±2%). All studied heavy metals were removed from aqueous solutions when the biosorbent dosage increased to 0.6% by dead biomass of A26 isolate. This confirmed the potential use of Streptomyces albus A26 as an inexpensive and efficient technology for removal of Cd²⁺ and Mn². The isolate A33 was recorded as high percentage also which was removed 68.3 %, and 69.4 % from Cd²⁺, and Mn²⁺ respectively and phylogenetic data of strain concluded that it was N. vermiculate under accession numbers MZ229469.1 in Gene Bank. The results of the dead biomass of A33 N.vermiculate for Cd²⁺, and Mn²⁺ under the optimized conditions for studied heavy metals were removed from aqueous solutions (Cd²⁺ 76.2 ± 1.17%, Mn^{+ 2} 72.6 ± 0.3%). The isolates A26 and A33 showed high removing effect for some aflatoxins (AFG1- AFG2-AFB1-AFB2) extracted from fungi group (Aspergillus flavus and Aspergillus parasiticus) under the optimized conditions (pH-8 at 40°C for 3 hours with 0.3% biosorbent dosage).

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