National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
This study was conducted to determine the lipid producing potential of microbial isolates from different soil samples from Ekiti State University. The media used were sterilized in an autoclave at 1210C for 15minutes, while serial dilution; isolation of colonies; identification and characterization of isolates were carried out from five different soil samples namely: kitchen effluent soil, cultivated, uncultivated soils, soil from dump site and palm oil spill site. The results showed that different fungi which include; Fusarium sp, Scopulariopsis sp, Verticullium sp, Aspergillus sp, Geotrichum sp, Trychophyton sp were detected from different soil samples. Bacterial species Enterobacter sp., Bacillus cereus were from cultivated soil while Bacillus sp., Escherichia coli, Staphylococcus aureus were detected from kitchen effluent soils respectively. Bacterial species Serratia marcescens, Bacillus cereus and Enterobacter sp. were isolated from uncultivated soil. Soil from dump site Escherichia coli and Bacillus cereus were detected and from palm oil spill site Bacillus sp and Staphylococcus aureus were detected. Fungal isolates screened for lipid content indicated only four fungal produced lipids with the highest production from isolate SDS 10 (7.98 g/L) and least in CS1(1.13 g/L). Gas Chromatography-MS showed that caproic acid had the highest concentration in the fungal isolates. The selected fungal isolates were identified using molecular genotypic identification via the 18SrRNA gene sequencing as Purpureocillium lilacinum, Fusarium oxysporum, Aspergillus flavus and Rhizopus oryzae. The best carbon source for lipid production for the fungal isolates was glucose (8.32 g/L) and least in starch (3.33 g/L). The highest (8.45 g/L) lipid production was recorded in fermentation medium supplemented with sodium nitrate from Fusarium oxysporum with the least (2.46 g/L) in the medium supplemented with peptone from Rhizopus oryzae. In conclusion, microorganisms identified from this study could be used to produce large amount of lipids for biofuel production.
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