National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The extensive use of organophosphate pesticides in cotton agroecosystems have caused long-term contamination of soil and there is a need to identify robust microbial communities which can survive and transform xenobiotic organic substances. The current research examined Natural diversity and functional potential of actinobacteria that were isolated in a study of pesticide-contaminated cotton soils in Beed district, India. Eight y-eight soil samples exhibited 73 actinobacterial isolates, giving a turn-over of 82.95%, and a great deal of spatial variability across sites. Screening at 500 ppm showed that there was an extensive adaptive resistance, with 19.18% of the isolates having a broad-spectrum resistance and 65.75% having a three-or-more pesticide resistance, showing prevalence of multi-pesticide resistance phenotypes. Nonetheless, when treated to high stress (1000 ppm), the tolerance decreased drastically as only 6.85% of the isolates were viable with all pesticides, which indicated close concentration-dependent toxicity as well as metabolic limitations. Phenotypic and biochemical characterization identified them to associate with actinobacterial taxa having elevated metabolic flexibility. Findings of molecular identification of the most effective isolate (PTA-52) showed that it has 99-99.6% similarity to Nocardiopsis, and phylogenetic analysis was observed to have a strain-level divergence, which is indicative of adaptations evolution due to big data changes pesticides. The results collectively indicate that the financially key actinobacterial community is present in cotton soils exposed to pesticides and that a small subset of the highly tolerant strains have great potential in high-load bioremediation systems.
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