National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
1Department of Veterinary Microbiology, College of Veterinary Science and A.H., Kamdhenu University, Navsari- 396450 (Gujarat), India
2National Centre for Microbial Resource Pune, India
To overcome the problems associated with identifying bacteria. The matrix-assisted laser desorption ionization (MALDI) technology was co invented by Franz Hillenkamp and Michael Karas in the mid-1980s. In this work, 77 isolates of avian pathogenic E. coli were analyzed by the MALDI-TOF MS method. Total 77 bacterial isolates were identified as E. coli on the basis on their mass to charge ratio (m/z). The strain showing ≥1.7 log value with strain in database were confirmed as the member of that genus and strains showing ≥2.0 log values were confirmed as the member of that species. Good quality MALDI-TOF MS spectra were generated for all E. coli strains in this study.
Croxatto, A., Prodhom, G., and Greub, G. (2012). Applications of MALDI-TOF Mass Spectrometry in clinical diagnostic Microbiology. FEMS Microbiology Review, 36: 380-407.
Fenselau, C., and Demirev, P. A. (2001). Characterization of intact microorganisms by MALDI TOF Mass Spectrometry. Mass Spectrometry Review, 20: 157-171
Karas, M., and Hillenkamp, F. (1988). Laser desorption ionization of proteins with molecular masses exceeding 10000 Daltons. Analytical Chemotherapy, 60: 2299 –301.
Rahi, P., Sharma, O. P., and Shouche, Y. S. (2016). Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass-Spectrometry (MALDI-TOF MS) based microbial identifications: challenges and scopes for microbial ecologists. Frontiers in Microbiology. 7: 1359.
Ryzhov V, Fenselau, C. (2001). Characterization of the protein subset desorbed by MALDI from whole bacterial cells. Anal Chem. 15; 73(4): 746-50. https://doi.org/10.1021/ac0008791.
Suarez, S., Ferroni, A., Lotz, A., Jolley, K.A., Guérin, P., Leto, J., Dauphin, B., Jamet, A., Maiden, M., Nassif, X., and Armengau, J. (2013). Ribosomal proteins as biomarkers for bacterial identification by mass spectrometry in the clinical microbiology laboratory. Journal of Microbiological Methods, 94: 390-396.
Shell, W. S., Sayed, M. L., Gad, A. M., Gamal, F. E., Khedr, A. A., Samy, A. A., and. Ali, A. M. (2017). Matrix-Assisted Laser Desorption-Ionization-Time-of-Flight Mass Spectrometry as a reliable proteomic method for characterization of Escherichia coli and Salmonella isolates. Veterinary World, 10: 1083-93.
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