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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles Volume : 15, Issue : 9, September, 2026

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2026.15(9) : 161-172
DOI : https://doi.org/10.20546/ijcmas.2026.1509.018


Phenology-Guided Foliar Bioregulation of Guava under Moisture Deficit: Physiological Mechanisms and Critical Developmental Windows

Sourabh S. Surve1, Jitendrakumar H. Kadam2*, Machhindra G. Agale1,  Dhananjay D. Nangare3, Ganesh S. Shinde1, Hrishikesh B. Thube1, Tejas. S. Patil1, Abhishek4 and Pandurang. S. Lomate5
1Department of Horticulture, Dr. Sharadchandra Pawar College of Agriculture, Baramati, Maharashtra, India
2School of Edaphic Stress Management, ICAR-NIASM, Malegaon, Baramati, Maharashtra, India
3School of Drought Stress Management, ICAR-National Institute of Abiotic Stress Management, Malegaon, Baramati, Maharashtra, India
4Department of Horticulture, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani, Maharashtra, India
5Department of Agronomy, College of Agriculture, Latur, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani, Maharashtra, India
*Corresponding author
Abstract:

Guava (Psidium guajava L.) is an important fruit crop whose growth, flowering, fruit setting and fruit development are strongly influenced by water availability. Moisture deficit can disturb plant water status, stomatal behavior, canopy temperature, chlorophyll stability, photosynthesis and antioxidant balance, resulting in reduced physiological performance during sensitive developmental stages. Guava phenology provides a useful framework for identifying these stage-specific responses and for improving the timing of crop-management practices. This review summarizes the major phenological stages of guava and discusses their relationship with moisture-deficit stress and foliar bioregulation. Particular attention is given to three important developmental windows: early bud development, flower-bud appearance or reproductive transition and the petal fall–fruit-set transition. The potential roles of salicylic acid, melatonin and glutathione in supporting stress tolerance through signaling, osmotic adjustment, antioxidant defense and protection of photosynthetic processes are also discussed. The review highlights that foliar interventions based on actual phenological stages may be more biologically meaningful than application based only on fixed calendar date days. However, further field validation is required to identify suitable bioregulator, dose and phenological-stage combinations under different cultivars, seasons and moisture conditions. Phenology-guided foliar management may provide a useful framework for investigating abiotic stress tolerance, water-use efficiency and sustainable guava production.


Keywords: Guava; Phenology; Moisture deficit; Salicylic acid; Melatonin; Glutathione


References:

Abrar MM, Sohail M, Saqib M, Akhtar J, Abbas G, Wahab HA, Mumtaz MZ, Mehmood K, Memon MS, Sun N, Xu M. Interactive salinity and water stress severely reduced the growth, stress tolerance, and physiological responses of guava (Psidium guajava L.). Scientific Reports. 2022; 12: 18952. https://doi.org/10.1038/s41598-022-22602-5.

Alfia MA, Vasugi C, Honnabyraiah MK, Adiga JD, Shivapriya M, Vincent L. Phenological stages of wild species and cultivated species of guava (Psidium guajava L.). International Journal of Pure & Applied Bioscience. 2017; 5(6): 464–474.

Ali A, Kant K, Kaur N, Gupta S, Jindal P, Gill SS, Naeem M. Salicylic acid: Homeostasis, signalling and phytohormone crosstalk in plants under environmental challenges. South African Journal of Botany. 2024; 169: 314–335.

Altaf MA, Shahid R, Lal P, Ahmad R, Zulfiqar F, Kumar A, Hayat F, Kumar R, Lal MK, Naz S, Tiwari RK. Current understanding of boosting power of salicylic acid for abiotic stress tolerance in horticultural crops. South African Journal of Botany. 2023; 163: 285–293.

Alves JE, Freitas BM. Pollination requirements of guava. Ciencia Rural. 2007; 37(5): 1281–1286. https://doi.org/10.1590/S0103-84782007000500010.

Cho SB, Soleh HM, Choi JW, Hwang WH, Lee H, Cho YS, Cho BK, Kim MS, Baek I, Kim G. Recent methods for evaluating crop water stress using AI techniques: A review. Sensors. 2024; 24(19): 6313. https://doi.org/10.3390/s24196313.

El-Buluk RE, Babiker EE, El Tinay AH. Biochemical and physical changes in fruits of four guava cultivars during growth and development. Food Chemistry. 1995; 54(3): 279–282. https://doi.org/10.1016/0308-8146(95)00047-M.

Fan S, Xiong T, Lei Q, Tan Q, Cai J, Song Z, Yang M, Chen W, Li X, Zhu X. Melatonin treatment improves postharvest preservation and resistance of guava fruit (Psidium guajava L.). Foods. 2022; 11(3): 262. https://doi.org/10.3390/foods11030262.

Foyer CH, Noctor G. Ascorbate and glutathione: The heart of the redox hub. Plant Physiology. 2011; 155(1): 2–18. https://doi.org/10.1104/pp.110.167569.

Galindo A, Collado-Gonzalez J, Grinan I, Corell M, Centeno A, Martin-Palomo MJ, Giron IF, Rodriguez P, Cruz ZN, Memmi H, Carbonell-Barrachina AA, Hernández F, Torrecillas A, Moriana A, Lopez-Perez D. Deficit irrigation and emerging fruit crops as a strategy to save water in Mediterranean semiarid agro-systems. Agricultural Water Management. 2018; 202: 311–324.

Gomasta J, Sarker BC, Haque MA, Anwari A, Mondal S, Uddin MS. Pruning techniques affect flowering, fruiting, yield and fruit biochemical traits in guava under transitory sub-tropical conditions. Heliyon. 2024; 10(9): e30064. https://doi.org/10.1016/j.heliyon.2024.e30064.

Hasanuzzaman M, Nahar K, Anee TI, Fujita M. Glutathione in plants: Biosynthesis and physiological role in environmental stress tolerance. Physiology and Molecular Biology of Plants. 2017; 23(2): 249–268.

Hayat Q, Hayat S, Irfan M, Ahmad A. Effect of exogenous salicylic acid under changing environment: A review. Environmental and Experimental Botany. 2010; 68(1): 14–25. https://doi.org/10.1016/j.envexpbot.2009.08.005.

Himanshu, Sharma S, Rana VS, Ankit, Thakur V, Kumar A, Prachi, Thakur S, Sharma N. Unlocking the sustainable role of melatonin in fruit production and stress tolerance: A review. CABI Agriculture and Bioscience. 2024; 5: 103.

Jat R, Singh VP, Ali Abed S, Al-Ansari N, Singh PK, Vishwakarma DK, Choudhary A, Al-Sadoon MK, Popat RC, Jat SK. Deficit irrigation scheduling with mulching and yield prediction of guava (Psidium guajava L.) in a subtropical humid region. Frontiers in Environmental Science. 2022; 10: 1044886.

Jayswal DK, Sharma DP, Dwevedi AK, Sharma TR. Pruning and integrated nutrient management for growth and yield of guava (Psidium guajava). The Indian Journal of Agricultural Sciences. 2019; 89(9): 1477–1481. https://doi.org/10.56093/ijas.v89i9.93493.

Jindal P, Kant K, Kaur N, Gupta S, Ali A, Naeem M. Melatonin: Discovery, biosynthesis, phytohormones crosstalk, and roles in agricultural crops under abiotic stress conditions. Environmental and Experimental Botany. 2024; 226: 105942. https://doi.org/10.1016/j.envexpbot.2024.105942.

Kashyap S, Prakash P, Barman K. Exogenous melatonin treatment delays senescence of guava fruit by modulating antioxidant activities. Applied Fruit Science. 2025; 67(5): 387. https://doi.org/10.1007/s10341-025-01622-1.

Khan FI, Akhtar S, Qamar M, Ismail T, Saeed W, Esatbeyoglu T, Jafari SM. A comprehensive review on guava: Nutritional profile, bioactive potential, and health-promoting properties of its pulp, peel, seeds, pomace and leaves. Trends in Food Science & Technology. 2025; 156: 104822.

Koti S, Ahlawat TR, Tandel BM, Patel AI, Niyaria R, Tank RV, Pandey AK, Zala VR. Variability in floral morphology, flowering behaviour and fruit set among guava (Psidium guajava L.) genotypes. Journal of Applied Horticulture. 2026; 28(2): 301–306. https://doi.org/10.37855/jah.2026.v28i02.47.

Lacerda CN, Lima GS, Soares LAA, Silva SS, Queiroz VF, Gheyi HR, Ferreira JTA, Almeida FS, Sousa VFO. Deficit irrigation and ascorbic acid in guava cultivation at different development stages. Ciencia Rural. 2026; 56(5): e20250078. https://doi.org/10.1590/0103-8478cr20250078.

Lakhawat SS, Sharma V, Singh TK, Patil P, Priyadevi S, Gutam S. Effects of pan evaporation-based drip irrigation levels on performance of guava grown in Udaipur and Rewa regions of India. Journal of Agrometeorology. 2024; 26(1): 69–73. https://doi.org/10.54386/jam.v26i1.2306.

Manjunath BL, Ramesha GK, Gutam S. Phenology-based irrigation management in guava orchards. Journal of Horticultural Sciences. 2024; 19(2). https://doi.org/10.24154/jhs.v19i2.2351.

Manzoor MA, Xu Y, Lv Z, Xu J, Wang Y, Sun W, Liu X, Wang L, Wang J, Liu R, Whiting MD, Jiu S, Zhang C. Melatonin: A multi-functional regulator of fruit crop development and abiotic stress response. Scientia Horticulturae. 2023; 321: 112282.

Menaka M, Asrey R, Meena NK, Vargheese E, Sethi S, Vinod BR, Ahamad S, Goswami AK. Effect of melatonin on biochemical changes, antioxidant system and oxidative membrane damage of Indian guava (cv. Barafkhana) during cold storage. South African Journal of Botany. 2024; 169: 95–108. https://doi.org/10.1016/j.sajb.2024.04.007.

Mercado-Silva E, Benito-Bautista P, García-Velasco MA. Fruit development, harvest index and ripening changes of guavas produced in central Mexico. Postharvest Biology and Technology. 1998; 13(2): 143–150. https://doi.org/10.1016/S0925-5214(98)00003-9.

Mondal K, Malhotra SP, Jain V, Singh R. Oxidative stress and antioxidant systems in guava (Psidium guajava L.) fruits during ripening. Physiology and Molecular Biology of Plants. 2009; 15(4): 327–334. https://doi.org/10.1007/s12298-009-0037-3.

Nithishni J, Rao V, Chinnaiyan V, Reddy BA, Hugar P. Assessment of floral phenology and pollen quality in guava (Psidium guajava L.) genotypes. Journal of Advances in Biology & Biotechnology. 2026; 29(1): 8–18. https://doi.org/10.9734/jabb/2026/v29i13502.

Noctor G, Mhamdi A, Chaouch S, Han Y, Neukermans J, Marquez-Garcia B, Queval G, Foyer CH. Glutathione in plants: An integrated overview. Plant, Cell & Environment. 2012; 35(2): 454–484. https://doi.org/10.1111/j.1365-3040.2011.02400.x.

Noctor G, Cohen M, Trémulot L, Châtel-Innocenti G, Van Breusegem F, Mhamdi A. Glutathione: A key modulator of plant defence and metabolism through multiple mechanisms. Journal of Experimental Botany. 2024; 75(15): 4549–4572. https://doi.org/10.1093/jxb/erae194.

Patel RK, Maiti CS, Deka BC, Deshmukh NA, Verma VK, Nath A. Physical and biochemical changes in guava (Psidium guajava L.) during various stages of fruit growth and development. International Journal of Agriculture, Environment and Biotechnology. 2015; 8(1): 75–82.

Rai GK, Kumar P, Choudhary SM, Singh H, Adab K, Kosser R, Magotra I, Kumar RR, Singh M, Sharma R, Corrado G, Rouphael Y. Antioxidant potential of glutathione and crosstalk with phytohormones in enhancing abiotic stress tolerance in crop plants. Plants. 2023; 12(5): 1133.

Rivas-San Vicente M, Plasencia J. Salicylic acid beyond defence: Its role in plant growth and development. Journal of Experimental Botany. 2011; 62(10): 3321–3338. https://doi.org/10.1093/jxb/err031.

Roque IA, Soares LAA, Lima VLA, Sousa VFO, Lima GS, Gheyi HR, Dantas MV, Ferreira JTA, Torres RAF, Silva STA. Foliar application of salicylic acid mitigates water deficit in guava. Revista Brasileira de Engenharia Agrícola e Ambiental. 2025; 29(5): e288437.

Sahoo J, Tarai RK, Sethy BK, Sahoo AK, Swain SC, Dash D. Flowering and fruiting behaviour of some guava genotypes under East and South East Coastal Plain Zone of Odisha, India. International Journal of Current Microbiology and Applied Sciences. 2017; 6(11): 3902–3911.

Salazar DM, Melgarejo P, Martínez R, Martínez JJ, Hernández F, Burguera M. Phenological stages of the guava tree (Psidium guajava L.). Scientia Horticulturae. 2006; 108(2): 157–161.

Santhoshkumar GM, Bhowmick N, Chakraborty A, Dey AN, Ghosh A, Dutta P, Deb P, Chamling N, Sherpa NT, Senjam BD. Effect of shoot pruning on growth, flowering and fruiting characteristics of different guava (Psidium guajava L.) cultivars. International Journal of Fruit Science. 2022; 22(1): 686–695. https://doi.org/10.1080/15538362.2022.2109094.

Selvaraj Y, Pal DK, Edward Raja M, Rawal RD. Changes in chemical composition of guava fruits during growth and development. Indian Journal of Horticulture. 1999; 56(1): 10–18.

Sharma S, Sehrawat SK, Saran PL. Comparative performance of superior guava genotypes in Northern India: Flowering and fruit set. Indian Journal of Horticulture. 2011; 68(2): 193–196.

Sharma S, Sehrawat SK, Sharma KD. Studies on time and duration of flowering, floral bud development and morphology of guava (Psidium guajava L.) under semi-arid region of India. International Journal of Current Microbiology and Applied Sciences. 2017; 6(12): 4176–4186.

Sharma S, Sharma G, Srivastav M, Pal R, Singh A, Sharma S, Abrol G, Choudhary R, Kumar A. Codification and description of growth stages in guava (Psidium guajava L.) using the extended BBCH scale. Scientific Reports. 2026. https://doi.org/10.1038/s41598-026-64870-5.

Singh A, Reddy KS, Singh J. Phenological stages of guava-A review. International Journal of Current Microbiology and Applied Sciences. 2020; 9(12): 736–744.

Singh G, Rajan S, Pandey D, Singh AK. Effect of soil-moisture stress on water relation by plant and cropping behaviour in guava (Psidium guajava). The Indian Journal of Agricultural Sciences. 1997; 67(7): 303–306.

Singh VK, Ravishankar H, Singh A, Soni MK. Pruning in guava (Psidium guajava) and appraisal of consequent flowering phenology using modified BBCH scale. The Indian Journal of Agricultural Sciences. 2015; 85(11): 1472–1476.

Usman M, Bokhari SAM, Fatima B, Rashid B, Nadeem F, Sarwar MB, Nawaz-ul-Rehman MS, Shahid M, Ayub CM. Drought stress mitigating morphological, physiological, biochemical and molecular responses of guava (Psidium guajava L.) cultivars. Frontiers in Plant Science. 2022; 13: 878616.

Vadez V, Grondin A, Chenu K, Henry A, Laplaze L, Millet EJ, Carminati A. Crop traits and production under drought. Nature Reviews Earth and Environment. 2024; 5: 211–225. https://doi.org/10.1038/s43017-023-00514-w.

Wang P, Sun X, Li C, Wei Z, Liang D, Ma F. Long-term exogenous application of melatonin delays drought-induced leaf senescence in apple. Journal of Pineal Research. 2013; 54(3): 292–302. https://doi.org/10.1111/jpi.12017.

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How to cite this article:

Sourabh S. Surve, Jitendrakumar H. Kadam, Machhindra G. Agale and Ganesh S. Shinde, Thube H. B. and Patil T. S. 2026. Phenology-Guided Foliar Bioregulation of Guava under Moisture Deficit: Physiological Mechanisms and Critical Developmental Windows. Int.J.Curr.Microbiol.App.Sci. 15(9): 161-172 doi: https://doi.org/10.20546/ijcmas.2026.1509.018
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